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## T Spot TB Test
The T Spot TB Test is a blood test used for tuberculosis (TB) screening. It detects effector T cells that have been specifically activated by Mycobacterium tuberculosis (MTB) antigens. The test is performed using the enzyme-linked immunospot (ELISPOT) methodology to enumerate M. tuberculosis-sensitized T cells by capturing interferon-gamma in the vicinity of T cells. This test is accurate across patient populations, including immunocompromised and BCG-vaccinated individuals, with a sensitivity and specificity of over 95%[2].
## Indications of the Test
The T Spot TB Test is intended for use as an aid in the diagnosis of MTB infection[1].
## Patient Preparation/Instructions
Patients are required to follow the instructions provided by the healthcare provider. The procedures for sample collection and preparation for the T Spot TB Test are different from those for other TB tests. Whole blood samples should be collected into specific blood collection tubes and the tubes should be shaken to allow mixing of the heparin with the blood[2].
## Typical Reference Values
The interpretation of the T Spot TB Test results involves subtracting the spot count in the nil control well from the spot count in each of the panels, according to a specific algorithm[2].
## Turnaround Time
The typical turnaround time for the T Spot TB Test is 1-3 days[1].
To order the test, click the “Order Test” button and add it to the cart.
Citations:
[1] Request Rejected-Code Aeon-Q https://testdirectory.questdiagnostics.com/test/test-detail/37737/t-spottb?cc=MASTER
[2] A2.3 T-SPOT.TB – step by step https://tbksp.org/en/node/2450
