What is the TPHA test?
The TPHA (Treponema Pallidum Hemagglutination Assay) test is a blood test used to detect antibodies against Treponema pallidum, the bacterium that causes syphilis. This test helps confirm whether a person has been exposed to syphilis by identifying specific antibodies in the blood. It is commonly used as a confirmatory test for syphilis, a sexually transmitted infection that can have serious health effects if left untreated.
Reasons for performing the TPHA Test
Doctors may recommend the TPHA test if you:
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Have symptoms suggestive of syphilis, such as painless sores (chancres), rashes, or swollen lymph nodes.
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Have been exposed to a sexual partner diagnosed with syphilis.
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Need routine screening, for example, during pregnancy or as part of sexual health check-ups.
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They are being monitored after syphilis treatment to check for antibodies.
- A confirmatory test after a positive VDRL/PRP test
Syphilis can present with a wide range of symptoms or sometimes none at all (latent syphilis), so testing is important for early detection and treatment.
Patient Preparation/Instructions – Any preparations needed before the test
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No special preparation is required. You do not need to fast or change your diet before the TPHA test
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Wear a shirt with sleeves that can be easily rolled up, as a blood sample will be taken from your arm.
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If your doctor has ordered other tests that require fasting, follow those instructions.
Normal result ranges
Test Name | Normal Result |
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Treponema Pallidum Hemagglutination (TPHA) | Negative |
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Negative: No antibodies to syphilis detected (normal result)5.
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Positive: Antibodies to syphilis are present, suggesting current or past infection. Further evaluation by your doctor is needed to interpret positive results.
Waiting time for results
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Results are typically available within 6 hours after your blood sample is collected.
How to order the test – Step-by-step guide for ordering online via Labtestzote
- Select the test and add it to your cart.
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Fill in your details and choose your preferred sample collection method:
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Visit a Labtestzote partner laboratory in Nairobi (Hurlingham)
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Book a home sample collection (if available)
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Come for your blood sample collection at the scheduled time, or walk in at any time during working hours
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Receive your results by email or through your Labtestzote account, usually within 1–2 days.
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Consult your doctor for interpretation and next steps.
Labtestzote serves clients in Nairobi and across Kenya, making it easy and convenient to get tested for syphilis and other health conditions.