What is the TPHA test?
The TPHA (Treponema Pallidum Hemagglutination Assay) test is a blood test used to detect antibodies against Treponema pallidum, the bacterium that causes syphilis. This test helps confirm whether a person has been exposed to syphilis by identifying specific antibodies in the blood. It is commonly used as a confirmatory test for syphilis, a sexually transmitted infection that can have serious health effects if left untreated.
Reasons for performing the TPHA Test
Doctors may recommend the TPHA test if you:
Have symptoms suggestive of syphilis, such as painless sores (chancres), rashes, or swollen lymph nodes.
Have been exposed to a sexual partner diagnosed with syphilis.
Need routine screening, for example, during pregnancy or as part of sexual health check-ups.
They are being monitored after syphilis treatment to check for antibodies.
- A confirmatory test after a positive VDRL/PRP test
Syphilis can present with a wide range of symptoms or sometimes none at all (latent syphilis), so testing is important for early detection and treatment.
Patient Preparation/Instructions – Any preparations needed before the test
No special preparation is required. You do not need to fast or change your diet before the TPHA test
Wear a shirt with sleeves that can be easily rolled up, as a blood sample will be taken from your arm.
If your doctor has ordered other tests that require fasting, follow those instructions.
Normal result ranges
| Test Name | Normal Result |
|---|---|
| Treponema Pallidum Hemagglutination (TPHA) | Negative |
Negative: No antibodies to syphilis detected (normal result)5.
Positive: Antibodies to syphilis are present, suggesting current or past infection. Further evaluation by your doctor is needed to interpret positive results.
Waiting time for results
Results are typically available within 6 hours after your blood sample is collected.
How to order the test – Step-by-step guide for ordering online via Labtestzote
- Select the test and add it to your cart.
Fill in your details and choose your preferred sample collection method:
Visit a Labtestzote partner laboratory in Nairobi (Hurlingham)
Book a home sample collection (if available)
Come for your blood sample collection at the scheduled time, or walk in at any time during working hours
Receive your results by email or through your Labtestzote account, usually within 1–2 days.
Consult your doctor for interpretation and next steps.
Labtestzote serves clients in Nairobi and across Kenya, making it easy and convenient to get tested for syphilis and other health conditions.

